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Alteration of gonadotropin-releasing hormone receptor expression with the progression of prostate cancer in the Dunning rat adenocarcinoma sublines.

Tieva A, Bergh A, Damber JE

Institute of Surgical and Perioperative Sciences, Urology & Andrology, Umeå University, Umeå, Sweden. ase.tieva@onkologi.umu.se

Inhibitory effects of GnRH analogues on tumour growth in vitro suggests that such direct effects may be of importance also in vivo. However, the role of GnRH receptors (GnRH-R) in prostate tumour progression is largely unknown. The aim was therefore to investigate the variation of GnRH-R expression with prostate tumour progression using Dunning rat adenocarcinoma sublines representing different prostate tumour grades. GnRH-R levels were quantified in the rat dorsolateral (DLP) and Dunning sublines (PAP, AT-1, AT-2, AT-3, MatLyLu) using competitive RT-PCR and Western blot. The results showed that all Dunning sublines had significantly elevated GnRH-R mRNA expression levels compared with DLP. Comparison of GnRH-R mRNA levels between different tumour grades revealed no difference in mRNA expression. However, the anaplastic and highly metastatic AT-3 and MatLyLu tumours displayed a tendency for lower GnRH-R mRNA values than the non-metastatic tumour sublines. Our data demonstrate the expression of GnRH-R in normal rat DLP and in different Dunning sublines. However, GnRH-R seems not to be involved in tumour progression.

Published 3 August 2005 in Acta Oncol, 44(3): 299-303.
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